Journal: NPJ Vaccines

Article Title: Protective efficacy of the pan-fungal vaccine NXT-2 against vulvovaginal candidiasis in a murine model

doi: 10.1038/s41541-025-01171-4

Figure Lengend Snippet: IL-1β ( A ) and S100A8 ( B ) concentrations were assessed via ELISA. All data represents mean ± SEM. Statistical significance was assessed by using the Mann-Whitney test for IL-1β and S100A8 concentrations. C Tissue damage was assessed by measuring lactate dehydrogenase (LDH) activity in vaginal lavage fluid twenty-eight days post vaccination (28dpv) and five days post challenge (5dpc). Data represents mean ± SEM. Statistical significance was assessed by two-way ANOVA with Tukey correction (* P = 0.0111, *** P = 0.0007, **** P < 0.0001). D Quantification of inflammation in vaginal tissue from sham and NXT-2 immunized mice excised 5 days post challenge. Data represents mean ± SEM. Statistical significance was assessed by unpaired student t -test (**** P < 0.0001). Vaginal tissue excised following immunization and C. albicans challenge was stained with H&E (sham— E , NXT-2— G ) and the neutrophil specific marker anti-mouse Ly6G (sham— F , NXT-2— H ). Tissue sections were scanned at 40× objective magnification using the Aperio AT2 (Leica Biosystems). Image analysis and capture was performed using ImageScope (Leica Biosystems) at 20× digital zoom level.

Article Snippet: S100A8 : Utilizing the Mouse S100A8 ELISA kit (Rockland Immunochemicals), samples were diluted 1:100 and the manufacturer’s instructions were followed.

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Activity Assay, Staining, Marker

Journal: Acta biochimica Polonica

Article Title: Over-expression of EPB41L3 promotes apoptosis of human cervical carcinoma cells through PI3K/AKT signaling.

doi: 10.18388/abp.2020_5649

Figure Lengend Snippet: Figure 1. Expressions of EPB41L3 in human cervical cancers and cell lines. (A) expression of EPB41L3 in human cervical cancer (red) and normal cervical tissue (gray) was analyzed in the cervical cancer clinical sequencing database by TCGA. (B) analysis of prognos tic survival curve in cervical cancer with high expression and low expression of EPB41L3. (C) different expression levels of EPB41L3 in different stages of cervical diseases analyzed by immunohisto chemical staining, including chronic cervicitis, CIN I, CIN III, and cervical cancer; the expression level of EPB41L3 were divided into four levels, such as negative, +, ++ and +++.

Article Snippet: The antibodies were directed against EPB41L3 (Proteintech, USA), β-actin (Abcam, England), PI3K (Abcam, England), p-PI3K(phospho Y458, Abcam, England), AKT (Abcam, England), p-AKT (phospho T308, Abcam, England), BAX (Abcam, England), cleaved caspase-3 (Abcam, England), Bcl2 (Abcam, England), and GAPDH (Santa Cruz, USA).

Techniques: Expressing, Sequencing, Staining

Journal: Acta biochimica Polonica

Article Title: Over-expression of EPB41L3 promotes apoptosis of human cervical carcinoma cells through PI3K/AKT signaling.

doi: 10.18388/abp.2020_5649

Figure Lengend Snippet: Figure 2. Title for whole figure (A) relative expression of EPB41L3 in Ect/E6E7 and 5 cervical cancer cell lines including HeLa, C-4I, SiHa, C33A, and CaSki, by western blot. Densitometric analysis of Western blot from three independent experiments; bars represent means ± S.E.M. of EPB41L3 level nor malized to β-Actin. ***P<0.001. (B) relative expression of EPB41L3 in cervical cancer cell lines by RT-PCR. All data were normalized relative to the mRNA concentration for GAPDH and are presented as the mean ± S.E.M. (n=3).**P<0.01, ***P<0.001, ns, no significant differences, compared with the control. (C) expression of EPB41L3 in HeLa and SiHa after transfection with LvCtrl or LvEPB41L3. Densitometric analy sis of western blot from three independent experiments; bars represent means ± S.E.M. of EPB41L3 level normalized to β-Actin. **P<0.01, ***P<0.001, compared with the control. (D) expression of EPB41L3 in HeLa and SiHa after transfection with LvCtrl or LvEPB41L3 by west ern blot.

Article Snippet: The antibodies were directed against EPB41L3 (Proteintech, USA), β-actin (Abcam, England), PI3K (Abcam, England), p-PI3K(phospho Y458, Abcam, England), AKT (Abcam, England), p-AKT (phospho T308, Abcam, England), BAX (Abcam, England), cleaved caspase-3 (Abcam, England), Bcl2 (Abcam, England), and GAPDH (Santa Cruz, USA).

Techniques: Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Concentration Assay, Control, Transfection